| Immunopharmacol Immunotoxicol. 2012 Feb;34(1):84-8. |
| Sanggenon C and O inhibit NO production, iNOS expression and NF-κB activation in LPS-induced RAW264.7 cells.[Pubmed: 21612567] |
Sanggenone C and O, two Diels-Alder type adducts isolated from Morus alba, a plant has been used for the anti-inflammatory purpose in the Oriental medicine, were investigated for their effect on the NO production, iNOS expression and NF-κB activity.
METHODS AND RESULTS:
The inhibitory effects of Sanggenone C and O on the NF-κB activity were investigated in LPS-stimulated RAW264.7 cells by SEAP reporter assay.Sanggenon O showed stronger inhibition than the diastereomer Sanggenone C. Both compounds prevented the phosphorylation and degradation of IκBα protein.
CONCLUSIONS:
We demonstrated that Sanggenone C and O inhibited NO production and iNOS expression by suppressing NF-κB activity and IκBα activation. |
| Acta Pharmacol Sin. 2002 Feb;23(2):138-42. |
| Inhibitory effect and mechanism of action of sanggenon C on human polymorphonuclear leukocyte adhesion to human synovial cells.[Pubmed: 11866874] |
To examine the effect of Sanggenone C on human polymorphonuclear leukocyte (PMN) adhesion to human synovial cell(HSC), and explore its mechanism.
METHODS AND RESULTS:
Adhesion of PMN to HSC was measured by MTT colorimetry. Cell-ELISA and RT-PCR methods were used to examine the expression of adhesion molecules ICAM-1 and VCAM-1. Activation of nuclear factor-kappa B(NF-kappaB) was measured by electrophoretic mobility shift assays(EMSA) method. Sanggenone C effectively inhibited TNF-alpha (50 kU/L for 12 h) and IL-1beta (50 kU/L for 12 h) induced adhesion of PMN to HSC (IC50 27.29 nmol/L and 54.45 nmol/L, respectively) in a concentration-dependent manner. Adhesion molecule VCAM-1 surface protein and mRNA expression induced by TNF-alpha 50 kU/L were significantly inhibited by Sanggenone C, nevertheless, for ICAM-1 only surface protein expression being inhibited. The activation of NF-kappaB was also extensively inhibited by Sanggenone C.
CONCLUSIONS:
Sanggenone C inhibited TNF-alpha -stimulated PMN-HSC adhesion and expression of VCAM-1 by suppressing the activation of NF-kappaB. |
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