In vitro: |
Journal of Traditional Medicines, 2003, 20: 22-9. | Inhibitors of nitric oxide (NO) production in murine macrophage-like J774.1 cells from Brazilian propolis[Reference: WebLink] | METHODS AND RESULTS:
Water and MeOH extracts of Brazilian propolis showed dose-dependent inhibition toward nitric oxide (NO) production in lipopolysacchalide (LPS)-activated murine macrophage-like J774.1 cells. From the water extract, 17 phenolic compounds were isolated and among them 15 are new for the water extract of propolis. Moreover, methyl ρ-hydroxydihydrocinnamate (9) and 1-(4-hydroxyphenyl)butane-1,3-dione (11) were isolated, for the first time, from propolis.
CONCLUSIONS:
Labdane-type diterpenes, flavonoids and some phenolic compounds possessed potent NO inhibitory activity. Coniferyl aldehyde (23) and Dimeric coniferyl acetate (33) showed the strongest NO inhibition with IC_50 values of 18.0 and 27.1 μM, respectively, which were stronger than the positive control, N^G-monomethyl-L-arginine (L-NMMA ; IC_50, 44.5 μM). |
|