In vivo: |
Chem Biol Interact. 2014 Jun 5;216:9-16. | Long-term supplementation of umbelliferone and 4-methylumbelliferone alleviates high-fat diet induced hypertriglyceridemia and hyperglycemia in mice.[Pubmed: 24661945] | This study was conducted to evaluate the effects of umbelliferone (UF) and 4-Methylumbelliferone (mUF) on high-fat diet-induced hypertriglyceridemia and hyperglycemia in mice. METHODS AND RESULTS: The mice were assigned to normal control, high-fat control, and high-fat with UF or 4-Methylumbelliferone groups. For UF or mUF groups, the high-fat diet was supplemented with UF or 4-Methylumbelliferone at 0.02% (wt/wt) for 12weeks. Both UF and 4-Methylumbelliferone significantly decreased plasma triglyceride, free fatty acid and glucose levels, adipocyte size, white adipose tissue weights, and hepatic phosphatidate phosphohydrolase activity and significantly increased plasma adiponectin levels and hepatic fatty acid β-oxidation activity compared with the high-fat control group. UF and 4-Methylumbelliferone improved glucose intolerance and hepatic steatosis in the high-fat fed mice. Long-term high-fat diet intake induced an increase in hepatic CYP2E1 activity and lipid peroxide and cytosolic hydrogen peroxide contents and suppressed superoxide dismutase and glutathione peroxidase activities, which were reversed by UF and 4-Methylumbelliferone supplementation. CONCLUSIONS: These results indicate that UF and 4-Methylumbelliferone similarly ameliorate hypertriglyceridemia and hyperglycemia partly by modulating hepatic lipid metabolism and the antioxidant defense system along with increasing adiponectin levels. | J Comp Physiol B. 2014 Aug;184(6):699-708. | Antioxidative properties of 4-methylumbelliferone are related to antibacterial activity in the silkworm (Bombyx mori) digestive tract.[Pubmed: 24997539] | Umbelliferones have gained significant attention due to their tumor-inhibitory effects in vitro. This study was undertaken to examine the impact of umbelliferones in an invertebrate model organism, Bombyx mori, to assess the underlying antimicrobial activities via antioxidation in vivo. METHODS AND RESULTS: Oral administration of 4 mM 4-methylumbelliferone (4-MU), a model umbelliferone drug, in B. Mori larvae caused a rapid increase in reactive oxygen species, such as hydrogen peroxide (H2O2) and antimicrobial activity in the digestive tract. In addition, a significant increase in total antioxidant capacity as well as superoxide anion radical-inhibiting activity and reduced glutathione were detected. The antioxidant defense system was activated following induction of H2O2, resulting in a significant rise in catalase (50-66 %) and glutathione peroxidase (175 %) activities, which were helpful in defending digestive tract cells against oxidative injury. CONCLUSIONS: These results help in understanding the anticancer mechanism of 4-MU based on its antioxidation in organisms. |
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