| FEMS Microbiol Lett. 1992 Jul 1;73(1-2):187-90. |
| An extracellular enzyme from Fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin.[Pubmed: 1521768] |
Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and Phaseollidin.
METHODS AND RESULTS:
Two enzyme activities, kievitone hydratase and Phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electrophoresis. Eluates from successive slices taken from the native gel were assayed for both activities.
CONCLUSIONS:
Although they were not completely separated in the native gel, the activity profiles indicated that the two activities were distinct. The Km of Phaseollidin hydratase for Phaseollidin was approximately 7 microM. |
| Aust. J. Chem., 1974, 27(27):1607-11. |
| Phaseollidin, a Phytoalexin From Phaseolus vulgaris: Isolation, Physicochemical Properties and Antifungal Activity[Reference: WebLink] |
METHODS AND RESULTS:
The isolation of phaseollidin from diffusates of fungal-inoculated fruits of Phaseolus vulgaris is described. Physicochemical and antifungal properties of this compound are reported.
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)
