| Description: |
Citric acid is a natural preservative and food tartness enhancer, it is also an Al-chelating substance and a natural antioxidant. Citric acid (1-2 g/kg) can decrease brain lipid peroxidation and inflammation, liver damage, and DNA fragmentation. Citric acid has phytoremediation of heavy metal contaminated soil; citric acid can decrease the adsorption of both lead and cadmium. Dietary citric acid can effectively improve phytate P utilization in chicks. |
| Targets: |
TNF-α | Caspase | Antifection |
| In vitro: |
| Chemosphere. 2003 Feb;50(6):807-11. | | The role of citric acid on the phytoremediation of heavy metal contaminated soil.[Pubmed: 12688495] | Adsorption and hydroponics experiments were conducted to study the role of citric acid on the phytoremediation of heavy metal contaminated soil.
METHODS AND RESULTS:
The results show that addition of citric acid decreased the adsorption of both lead and cadmium, such an effect was bigger for cadmium than for lead. The decrease in the adsorption of Pb and Cd was mainly due to a decrease of pH in the presence of citric acid. The presence of citric acid could alleviate the toxicity of Pb and Cd to radish, and stimulate their transportation from root to shoot. The studies of heavy metal forms using sequential extraction demonstrated that lead was mainly existed as FHAC (a lower bioavailable form) in the root, while F(HCl) was the dominant form in the leaf. The addition of citric acid to the soil changed the concentration and relative abundance of all the forms. The detoxifying effect of citric acid to Pb in shoots might result from the transformation of higher toxic forms into lower toxic forms. Cadmium was mainly present as F(NaCl), therefore, it had higher toxicity than lead.
CONCLUSIONS:
The addition of citric acid increased the abundance of F(H2O) + F(NaCl), indicating that citric acid treatment could transform cadmium into more transportable forms. | | BMC Oral Health. 2014 Jun 23;14:77. | | Efficacy of citric acid denture cleanser on the Candida albicans biofilm formed on poly(methyl methacrylate): effects on residual biofilm and recolonization process.[Pubmed: 24957210] | It is well known that the use of denture cleansers can reduce Candida albicans biofilm accumulation; however, the efficacy of citric acid denture cleansers is uncertain. In addition, the long-term efficacy of this denture cleanser is not well established, and their effect on residual biofilms is unknown. This in vitro study evaluated the efficacy of citric acid denture cleanser treatment on C. albicans biofilm recolonization on poly(methyl methacrylate) (PMMA) surface.
METHODS AND RESULTS:
C. albicans biofilms were developed for 72 h on PMMA resin specimens (n = 168), which were randomly assigned to 1 of 3 cleansing treatments (CTs) overnight (8 h). CTs included purified water as a control (CTC) and two experimental groups that used either a 1:5 dilution of citric acid denture cleanser (CT5) or a 1:8 dilution of citric acid denture cleanser (CT8). Residual biofilms adhering to the specimens were collected and quantified at two time points: immediately after CTs (ICT) and after cleaning and residual biofilm recolonization (RT). Residual biofilms were analyzed by quantifying the viable cells (CFU/mL), and biofilm architecture was evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Denture cleanser treatments and evaluation periods were considered study factors. Data were analyzed using two-way ANOVA and Tukey's Honestly Significant Difference (HSD) test (α = 0.05).
Immediately after treatments, citric acid denture cleansing solutions (CT5 and CT8) reduced the number of viable cells as compared with the control (p < 0.01). However, after 48 h, both CT groups (CT5 and CT8) showed biofilm recolonization (p < 0.01). Residual biofilm recolonization was also detected by CLSM and SEM analysis, which revealed a higher biomass and average biofilm thickness for the CT8 group (p < 0.01).
CONCLUSIONS:
Citric acid denture cleansers can reduce C. albicans biofilm accumulation and cell viability. However, this CT did not prevent biofilm recolonization. | | Iranian Journal of Fisheries Sciences, 2010, 9(9):279-292. | | Inhibitory impacts of natural antioxidants (ascorbic and citric acid) and vacuum packaging on lipid oxidation in frozen Persian sturgeon fillets[Reference: WebLink] | This study was aimed to investigate effects of aqueous citric acid (CA) and ascorbic acid (AA)
on lipid oxidation in comparison with effect of vacuum packaging in order to find better
treatment to delay improper changes in the Persian sturgeon (Acipenser persicus) fillets during
frozen storage due to lipid oxidation.
METHODS AND RESULTS:
In this study traditional packaging, vacuum packaging,
ascorbic acid solution (0.5 %) and citric acid solution (0.5 %) were considered as treatments.
Rancidity development was measured by several biochemical indicators including Free Fatty
Acids, Peroxide values and Thiobarbituric acid. Also pH, expressible moisture and sensory
properties were measured during 6 months storage. Results showed that free fatty acid (FFA),
primary and secondary oxidation products of control samples were significantly higher than
those in other treatments (p<0.05). Also, expressible moisture and pH value of treated samples
were significantly lower than those in control (p<0.05). However both antioxidants (AA and CA)
extended shelf life of frozen fillets but rancidity development in CA treated samples was higher
than other samples during storage.
CONCLUSIONS:
Results showed that all three treatments had significant effect
on delaying lipid oxidation (p<0.05) but usage of AA and vacuum packaging had the best effect
on delaying lipid oxidation and increasing shelf-life of fillets (p<0.05). Thus the employment of
AA and vacuum packaging alone or in combination with other protective strategies is
recommended.
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| In vivo: |
| J Med Food. 2014 May;17(5):588-98. | | Citric acid effects on brain and liver oxidative stress in lipopolysaccharide-treated mice.[Pubmed: 24433072] | Citric acid is a weak organic acid found in the greatest amounts in citrus fruits. This study examined the effect of citric acid on endotoxin-induced oxidative stress of the brain and liver.
METHODS AND RESULTS:
Mice were challenged with a single intraperitoneal dose of lipopolysaccharide (LPS; 200 μg/kg). Citric acid was given orally at 1, 2, or 4 g/kg at time of endotoxin injection and mice were euthanized 4 h later. LPS induced oxidative stress in the brain and liver tissue, resulting in marked increase in lipid peroxidation (malondialdehyde [MDA]) and nitrite, while significantly decreasing reduced glutathione, glutathione peroxidase (GPx), and paraoxonase 1 (PON1) activity. Tumor necrosis factor-alpha (TNF-α) showed a pronounced increase in brain tissue after endotoxin injection. The administration of citric acid (1-2 g/kg) attenuated LPS-induced elevations in brain MDA, nitrite, TNF-α, GPx, and PON1 activity. In the liver, nitrite was decreased by 1 g/kg citric acid. GPx activity was increased, while PON1 activity was decreased by citric acid. The LPS-induced liver injury, DNA fragmentation, serum transaminase elevations, caspase-3, and inducible nitric oxide synthase expression were attenuated by 1-2 g/kg citric acid. DNA fragmentation, however, increased after 4 g/kg citric acid.
CONCLUSIONS:
Thus in this model of systemic inflammation, citric acid (1-2 g/kg) decreased brain lipid peroxidation and inflammation, liver damage, and DNA fragmentation. |
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