| Biomed Chromatogr. 2015 Jan 26. doi: 10.1002/bmc.3420. |
| Simultaneous determination of senkyunolide I and senkyunolide H in rat plasma by LC-MS: application to a comparative pharmacokinetic study in normal and migrainous rats after oral administration of Chuanxiong Rhizoma extract.[Pubmed: 25620053] |
METHODS AND RESULTS:
A selective liquid chromatographic-mass spectrometric method has been developed and validated for simultaneous determination of senkyunolide I (SEI) and Senkyunolide H (SEH) from Chuanxiong Rhizoma in rat plasma. Plasma samples were extracted by liquid-liquid extraction with ethyl acetate and separated on a Kromasil C18 column (250 × 4.6 mm, 5 µm), with methanol-water (55:45, v/v) as mobile phase. The linear range was 0.05-25 µg/mL for SEI and 0.01-5.0 µg/mL for Senkyunolide H, with lower limits of quantitation of 0.05 and 0.01 µg/mL, respectively. Intra- and inter-day precision were within 10.0 and 9.8%, and the accuracies (relative errors) were <9.6 and 5.9%, with the mean extraction recoveries 81.0-86.6 and 80.5-85.0% for the two anayltes, respectively. The validated method was successfully applied to a comparative pharmacokinetic study of SEI and Senkyunolide H in normal and migrainous rats after oral administration of Chuanxiong Rhizoma extract.
CONCLUSIONS:
The results indicated that there were obvious differences between normal and migrainous rats in the pharmacokinetic behavior after oral administration of Chuanxiong Rhizoma extract. The absorption of SEI and Senkyunolide H were significantly increased in migrainous rats compared with normal rats. |
| Lishizhen Medicine & Materia Medica Research, 2003, 14(12): 738-9. |
| Effects of Ferulic Acid,Senkyunolide H and Senkyunolide I on Erythrocytes.[Reference: WebLink] |
To study the effects of Ferulic acid、Senkyunolide H and Senkyunolide I on erythrocytes deformability and aggregation.
METHODS AND RESULTS:
Deformability and aggregation of the dog RBC were evaluated through erythrocytes deformability experiment.
CONCLUSIONS:
Ferulic acid、Senkyunolide H and Senkyunolide I can reduce the metamorphose damage of the RBC caused by ConA. The aggregation experiment also has indicated that aggregation of the RBC can be alleviated by them. |
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