Info: Read More
  • 中药标准品生产商,产品定制服务
  • 柴胡皂苷D

    Saikosaponin D

    柴胡皂苷D
    产品编号 CFN99989
    CAS编号 20874-52-6
    分子式 = 分子量 C42H68O13 = 780.99
    产品纯度 >=98%
    物理属性 Powder
    化合物类型 Triterpenoids
    植物来源 The herbs of Bupleurum chinense DC.
    ChemFaces的产品在影响因子大于5的优秀和顶级科学期刊中被引用
    提供自定义包装
    产品名称 产品编号 CAS编号 包装 QQ客服
    柴胡皂苷D CFN99989 20874-52-6 10mg QQ客服:3257982914
    柴胡皂苷D CFN99989 20874-52-6 20mg QQ客服:3257982914
    柴胡皂苷D CFN99989 20874-52-6 50mg QQ客服:3257982914
    柴胡皂苷D CFN99989 20874-52-6 100mg QQ客服:3257982914
    存储与注意事项
    1. 在您收到产品后请检查产品。如无问题,请将产品存入冰霜并且样品瓶保持密封,产品可以存放长达24个月(2-8摄氏度)。

    2. 只要有可能,产品溶解后,您应该在同一天应用于您的实验。 但是,如果您需要提前做预实验,或者需要全部溶解,我们建议您将溶液以等分试样的形式存放在-20℃的密封小瓶中。 通常,这些可用于长达两周。 使用前,打开样品瓶前,我们建议您将产品平衡至室温至少1小时。

    3. 需要更多关于溶解度,使用和处理的建议? 请发送电子邮件至:service@chemfaces.com
    订购流程
  • 1. 在线订购
  • 请联系我们QQ客服

  • 2. 电话订购
  • 请拨打电话:
    027-84237683 或 027-84237783

  • 3. 邮件或传真订购
  • 发送电子邮件到: manager@chemfaces.com 或
    发送传真到:027-84254680

  • 提供订购信息
  • 为了方便客户的订购,请需要订购ChemFaces产品的客户,在下单的时候请提供下列信息,以供我们快速为您建立发货信息。
  •  
  • 1. 产品编号(CAS No.或产品名称)
  • 2. 发货地址
  • 3. 联系方法 (联系人,电话)
  • 4. 开票抬头 (如果需要发票的客户)
  • 5. 发票地址(发货地址与发票地址不同)
  • 发货时间
    1. 付款方式为100%预付款客户,我们将在确认收到货款后当天或1-3个工作日发货。

    2. 付款方式为月结的客户,我们承诺在收到订单后当天或1-3个工作日内发货。

    3. 如果客户所需要的产品,需要重新生产,我们有权告知客户,交货时间需要延期。
    ChemFaces的产品在许多优秀和顶级科学期刊中被引用

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    我们的产品现已经出口到下面的研究机构与大学,并且还在增涨
  • Heidelberg University (Germany)
  • University of Toronto (Canada)
  • Northeast Normal University Changchun (China)
  • Vin?a Institute of Nuclear Sciences (Serbia)
  • National Chung Hsing University (Taiwan)
  • University of Mysore (India)
  • Gyeongsang National University (Korea)
  • University of Perugia (Italy)
  • Monash University Malaysia (Malaysia)
  • Centralised Purchases Unit (CPU), B.I.T.S (India)
  • Sanford Burnham Prebys Medical Discovery Institute (USA)
  • Chinese University of Hong Kong (China)
  • Universiti Sains Malaysia (Malaysia)
  • FORTH-IMBB (Greece)
  • More...
  • 国外学术期刊发表的引用ChemFaces产品的部分文献
  • Appl Biol Chem2019, 62:46
  • Phytother Res.2018, 32(12):2551-2559
  • Molecules.2022, 27(5):1675
  • Process Biochemistry2019, 87:213-220
  • Front Immunol.2018, 9:2091
  • J Breast Cancer.2015, 18(2):112-118
  • Biosci Biotechnol Biochem.2020, 84(3):621-632
  • Food Sci Nutr.2019, 8(1):246-256
  • Pak J Pharm Sci.2018, 31:311-315
  • Phytomedicine.2015, 22(4):498-503
  • Journal of Functional Foods2021, 84:104581
  • Front Immunol.2023, 14:1240800.
  • Phytomedicine.2023, 117:154929.
  • Anal Biochem.2019, 569:10-15
  • TCI CO.2019, US20190151257A1
  • Arch Biochem Biophys.2018, 644:93-99
  • Biol Pharm Bull.2018, 41(11):1645-1651
  • J Cosmet Dermatol.2022, 21(1):396-402.
  • Fitoterapia.2022, 157:105130.
  • Cancers (Basel).2023, 15(1):37.
  • Asian J Beauty Cosmetol2022, 20(2):183-191
  • Front Nutr.2021, 8: 687851.
  • Molecules2021, 26(1),230
  • ...
  • 生物活性
    Description: Saikosaponin D, a calcium mobilizing agent (SERCA inhibitor), is also an agonist of the glucocorticoid receptor (GR),which has anti-cancer, anti-inflammatory, and neuroprotective effects. Saikosaponin D protects against acetaminophen-induced hepatotoxicity by inhibiting NF-κB and STAT3 signaling; it shows inhibitory effects on NF-κB activation and thereby on iNOS, COX-2 and pro-inflammatory cytokines.
    Targets: p53 | Bcl-2/Bax | p21 | CDK | NF-kB | STAT | SOD | HSP (e.g. HSP90) | Glucocorticoid Receptor | Calcium Channel | ATPase | AMPK | NOS | COX | NO | PGE | TNF-α | IL Receptor | SERCA inhibitor
    In vitro:
    Eur Rev Med Pharmacol Sci. 2014;18(17):2435-43.
    Saikosaponin-d inhibits proliferation of human undifferentiated thyroid carcinoma cells through induction of apoptosis and cell cycle arrest.[Pubmed: 25268087]
    Saikosaponin D is a triterpene saponin derived from Bupleurum falcatum. L and has been reported to exhibit a variety of pharmacological activities such as anti-bacterial, anti-virus and anti-cancer. The aim of the present study was to explore the effect of Saikosaponin D on the proliferation and apoptosis of human undifferentiated thyroid carcinoma.
    METHODS AND RESULTS:
    Three human anaplastic thyroid cancers cell lines were cultured in the presence of Saikosaponin D and their proliferation was measured by MTT assay. Cell apoptosis and cell cycle distribution were analyzed with flow cytometry. Western blot was performed to determine the proteins expression. The in vivo effect of Saikosaponin D was measured with an animal model. In vitro, MTT assay showed that Saikosaponin D treatment inhibited cell proliferation in three human anaplastic thyroid cancers cell lines ARO, 8305C and SW1736. In addition, Saikosaponin D promoted cell apoptosis and induced G1-phase cell cycle arrest as shown by flow cytometric analysis. On the molecular level, our results showed that saikosaponin-d treatment increased the expression of p53 and bax, and decreased the expression of Bcl-2. In addition, saikosaponin-d administration led to a significant up-regulation of p21 and down-regulation of CDK2 and cyclin D1. Xenografts tumorigenesis model demonstrated that Saikosaponin D markedly reduced the weight and volume of thyroid tumors in vivo.
    CONCLUSIONS:
    The present study suggested that Saikosaponin D might be a new potent chemopreventive drug candidate for human undifferentiated thyroid carcinoma through induction of apoptosis and cell cycle arrest.
    Int. Immunopharmacol., 2012 Sep;14(1):121-6.
    Saikosaponin a and its epimer saikosaponin d exhibit anti-inflammatory activity by suppressing activation of NF-κB signaling pathway.[Pubmed: 22728095 ]
    Saikosaponin a (SSa) and its epimer Saikosaponin D (SSd) are major triterpenoid saponin derivatives from Radix bupleuri (RB), which has been long used in Chinese traditional medicine for treatment of various inflammation-related diseases.
    METHODS AND RESULTS:
    In the present study, the anti-inflammatory activity, as well as the underlying mechanism, of SSa and SSd was investigated in lipopolysaccharide (LPS)-induced RAW264.7 cells. Our results demonstrated that both SSa and SSd significantly inhibited the expression of inducible nitric-oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW264.7 cells, and finally resulted in the reduction of nitric oxide (NO) and prostaglandin E(2) (PGE(2)). In addition, LPS-induced production of major pro-inflammatory cytokines: the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), was suppressed in a dose-dependent manner by the treatment of SSa or SSd in RAW264.7 cells. Further analysis revealed that both SSa and SSd could inhibit translocation of nuclear factor-κB (NF-κB) from the cytoplasm to the nucleus in the LPS-induced RAW264.7 cells. Furthermore, SSa and SSd exhibited significant anti-inflammatory activity in two different murine models of acute inflammation, carrageenan-induced paw edema in rats and acetic acid-induced vascular permeability in mice.
    CONCLUSIONS:
    In conclusion, SSa and SSd showed potent anti-inflammatory activity through inhibitory effects on NF-κB activation and thereby on iNOS, COX-2 and pro-inflammatory cytokines.
    2018 Mar;41(3):1357-1364.
    Estrogen receptor‑β‑dependent effects of saikosaponin‑d on the suppression of oxidative stress‑induced rat hepatic stellate cell activation[Pubmed: 29286085]
    Abstract Saikosaponin-d (SSd) is one of the major triterpenoid saponins derived from Bupleurum falcatum L., which has been reported to possess antifibrotic activity. At present, there is little information regarding the potential target of SSd in hepatic stellate cells (HSCs), which serve an important role in excessive extracellular matrix (ECM) deposition during the pathogenesis of hepatic fibrosis. Our recent study indicated that SSd may be considered a novel type of phytoestrogen with estrogen‑like actions. Therefore, the present study aimed to investigate the effects of SSd on the proliferation and activation of HSCs, and the underlying mechanisms associated with estrogen receptors. In the present study, a rat HSC line (HSC‑T6) was used and cultured with dimethyl sulfoxide, SSd, or estradiol (E2; positive control), in the presence or absence of three estrogen receptor (ER) antagonists [ICI‑182780, methylpiperidinopyrazole (MPP) or (R,R)-tetrahydrochrysene (THC)], for 24 h as pretreatment. Oxidative stress was induced by exposure to hydrogen peroxide for 4 h. Cell proliferation was assessed by MTT growth assay. Malondialdehyde (MDA), CuZn-superoxide dismutase (CuZn-SOD), tissue inhibitor of metalloproteinases-1 (TIMP-1), matrix metalloproteinase-1 (MMP-1), transforming growth factor-β1 (TGF-β1), hydroxyproline (Hyp) and collagen-1 (COL1) levels in cell culture supernatants were determined by ELISA. Reactive oxygen species (ROS) was detected by flow cytometry. Total and phosphorylated mitogen-activated protein kinases (MAPKs) and α-smooth muscle actin (α-SMA) were examined by western blot analysis. TGF-β1 mRNA expression was determined by RT-quantitative (q)PCR. SSd and E2 were able to significantly suppress oxidative stress‑induced proliferation and activation of HSC‑T6 cells. Furthermore, SSd and E2 were able to reduce ECM deposition, as demonstrated by the decrease in transforming growth factor‑β1, hydroxyproline, collagen‑1 and tissue inhibitor of metalloproteinases‑1, and by the increase in matrix metalloproteinase‑1. These results suggested that the possible molecular mechanism could involve downregulation of the reactive oxygen species/mitogen‑activated protein kinases signaling pathway. Finally, the effects of SSd and E2 could be blocked by co‑incubation with ICI‑182780 or THC, but not MPP, thus indicating that ERβ may be the potential target of SSd in HSC‑T6 cells. In conclusion, these findings suggested that SSd may suppress oxidative stress‑induced activation of HSCs, which relied on modulation of ERβ.
    制备储备液(仅供参考)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.2804 mL 6.4021 mL 12.8043 mL 25.6085 mL 32.0107 mL
    5 mM 0.2561 mL 1.2804 mL 2.5609 mL 5.1217 mL 6.4021 mL
    10 mM 0.128 mL 0.6402 mL 1.2804 mL 2.5609 mL 3.2011 mL
    50 mM 0.0256 mL 0.128 mL 0.2561 mL 0.5122 mL 0.6402 mL
    100 mM 0.0128 mL 0.064 mL 0.128 mL 0.2561 mL 0.3201 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    部分图片展示
    产品名称 产品编号 CAS编号 分子式 = 分子量 位单 联系QQ
    Borapetoside A; Borapetoside A CFN95658 100202-29-7 C26H34O12 = 538.6 10mg QQ客服:2056216494
    3,4-Seco-3-oxobisabol-10-ene-4,1-olide; 3,4-Seco-3-oxobisabol-10-ene-4,1-olide CFN89267 1564265-85-5 C15H24O3 = 252.35 5mg QQ客服:215959384
    瓜子金皂苷戊; Polygalasaponin E CFN95138 882664-72-4 C47H74O19 = 943.1 5mg QQ客服:1413575084
    Methyl 2alpha-hydroxyhardwickiate; Methyl 2alpha-hydroxyhardwickiate CFN89315 50428-93-8 C21H30O4 = 346.46 5mg QQ客服:1457312923

    信息支持


    公司简介
    订购流程
    付款方式
    退换货政策

    ChemFaces提供的产品仅用于科学研究使用,不用于诊断或治疗程序。

    联系方式


    电机:027-84237783
    传真:027-84254680
    在线QQ: 1413575084
    E-Mail:manager@chemfaces.com

    湖北省武汉沌口经济技术开区车城南路83号1号楼第三层厂房


    ChemFaces为科学家,科研人员与企业提供快速的产品递送。我们通过瑞士SGS ISO 9001:2008质量体系认证天然化合物与对照品的研发和生产