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  • 倒千里光碱

    Retrorsine

    倒千里光碱
    产品编号 CFN00407
    CAS编号 480-54-6
    分子式 = 分子量 C18H25NO6 = 351.40
    产品纯度 >=98%
    物理属性 Powder
    化合物类型 Alkaloids
    植物来源 The herbs of Senecio scandens
    ChemFaces的产品在影响因子大于5的优秀和顶级科学期刊中被引用
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    产品名称 产品编号 CAS编号 包装 QQ客服
    倒千里光碱 CFN00407 480-54-6 1mg QQ客服:2159513211
    倒千里光碱 CFN00407 480-54-6 5mg QQ客服:2159513211
    倒千里光碱 CFN00407 480-54-6 10mg QQ客服:2159513211
    倒千里光碱 CFN00407 480-54-6 20mg QQ客服:2159513211
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    ChemFaces的产品在许多优秀和顶级科学期刊中被引用

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    我们的产品现已经出口到下面的研究机构与大学,并且还在增涨
  • National Cancer Institute (USA)
  • Cornell University (USA)
  • Washington State University (USA)
  • Universitas Airlangga (Indonesia)
  • University of Eastern Finland (Finland)
  • University of Otago (New Zealand)
  • University of Vigo (Spain)
  • Tokyo Woman's Christian University (Japan)
  • VIT University (India)
  • Universidad de La Salle (Mexico)
  • Leibniz-Institut für Pflanzenbiochemie (IPB) (Germany)
  • Chulalongkorn University (Thailand)
  • Weizmann Institute of Science (Israel)
  • Subang Jaya Medical Centre (Malaysia)
  • More...
  • 国外学术期刊发表的引用ChemFaces产品的部分文献
  • Front Pharmacol.2017, 8:205
  • Molecules.2021, 26(2):E255.
  • Med Sci Monit.2019, 25:9499-9508
  • Phytomedicine.2019, 61:152813
  • Revista Brasileira de Farmacognosia2021, 31:794-804.
  • Polytechnic University of Catalonia2017, 105826
  • Evidence-based Compl.&Alternative Med.2023, 5417813
  • Adaptive Medicine 2020, 12(1): 4-10
  • Int Immunopharmacol.2023, 123:110572.
  • Molecules.2019, 24(11):E2044
  • Sci Rep. 2017, 12953(7)
  • Appl. Sci.2020, 10(23), 8729
  • JOTCSA.2023, 10(4); 893-902.
  • Phytother Res.2019, 33(5):1490-1500
  • Int Immunopharmacol.2021, 100:108073.
  • Molecules.2019, 24(6):E1177
  • Journal of Life Science2018, 917-922
  • Heliyon.2022, e12337.
  • J Ethnopharmacol.2017, 196:75-83
  • European Journal of Integrative Medicine2018, 20:165-172
  • Int J Mol Sci.2022, 23(13):7115.
  • Appl Biochem Biotechnol.2020, 190(2):732-744
  • Nutrients.2018, 10(10)
  • ...
  • 生物活性
    Description: Retrorsine selectively inhibits hepatocyte proliferation and following liver injury evokes small hepatocyte-like progenitor cells.OCT1 mediates the liver-specific uptake of Retrorsine, and plays an important role in Retrorsine-induced hepatotoxicity together with CYP3A4. Consequently, the OCT1 inhibitors could be applied to protect the liver from the toxicity of Retrorsine.
    Targets: P450 (e.g. CYP17)
    In vitro:
    Toxicology. 2014 Aug 1;322:34-42.
    Involvement of organic cation transporter 1 and CYP3A4 in retrorsine-induced toxicity.[Pubmed: 24799337]
    Retrorsine (RTS) is a hepatotoxic pyrrolizidine alkaloid present in plants of the Senecio genus. The present study is aimed at clarifying the role of organic cation transporters (OCTs) in the liver disposition of Retrorsine, and the coupling of OCT1 and cytochrome P450 (CYP) 3A4 in the hepatotoxicity of RTS.
    METHODS AND RESULTS:
    MDCK or LLC-PK1 cells stably expressing liver uptake or efflux transporters were used to investigate the interaction of Retrorsine with these transporters. Primary cultured rat hepatocytes (PCRH) and double-transfected MDCK-hOCT1-CYP3A4 cells were used to determine the contribution of OCT1 and CYP3A4 to the toxicity of Retrorsine. The results showed that Retrorsine inhibited the OCT1-mediated 1-methyl-4-phenylpyridinium (MPP(+)) uptake in MDCK-hOCT1 cells with the IC50 of 2.25±0.30μM. The uptake of Retrorsine in MDCK-hOCT1 cells and PCRH was significantly inhibited by OCT1 inhibitors, while hOCT3, human multidrug and toxin extrusion (hMATE) transporter 1, multidrug resistance 1 (MDR1), and breast cancer resistance protein (BCRP) showed weak or no obvious interaction with Retrorsine. The toxic effect of Retrorsine on the PCRH was attenuated by OCT1 inhibitors, quinidine and (+)-tetrahydropalmatine ((+)-THP). Compared to mock cells, MDCK-CYP3A4 cells showed a decrease in viability after being treated with Retrorsine. Furthermore, Retrorsine showed a more severe toxicity in the OCT1/CYP3A4 double-transfected cells compared to all other cells.
    CONCLUSIONS:
    Our data suggests that OCT1 mediates the liver-specific uptake of Retrorsine, and plays an important role in Retrorsine-induced hepatotoxicity together with CYP3A4. Consequently, the OCT1 inhibitors could be applied to protect the liver from the toxicity of Retrorsine.
    Hepatology. 2013 Mar;57(3):1215-24.
    Contribution of mature hepatocytes to small hepatocyte-like progenitor cells in retrorsine-exposed rats with chimeric livers.[Pubmed: 23080021]
    The potential lineage relationship between hepatic oval cells, small hepatocyte-like progenitor cells (SHPCs), and hepatocytes in liver regeneration is debated.
    METHODS AND RESULTS:
    To test whether mature hepatocytes can give rise to SHPCs, rats with dipeptidyl peptidase IV (DPPIV) chimeric livers, which harbored endogenous DPPIV-deficient hepatocytes and transplanted DPPIV-positive hepatocytes, were subjected to retrorsine treatment followed by partial hepatectomy (PH). DPPIV-positive hepatocytes comprised about half of the DPPIV chimeric liver mass. Tissues from DPPIV chimeric livers after retrorsine/PH treatment showed large numbers of SHPC clusters. None of the SHPC clusters were stained positive for DPPIV in any analyzed samples. Furthermore, serial sections stained for gamma-glutamyl-transpeptidase (GGT, a marker of fetal hepatoblasts) and glucose-6-phosphatase (G6Pase, a marker of mature hepatocytes) showed inverse expression of the two enzymes and a staining pattern consistent with a lineage that begins with GGT(+)/G6Pase(-) to GGT(-)/G6Pase(+) within a single SHPC cluster. Using double immunofluorescence staining for markers specific for hepatic oval cells and hepatocytes in serial sections, oval cell proliferations with CK-19(+)/laminin(+) and OV-6(+)/C/EBP-α(-) were shown to extend from periportal areas into the SPHC clusters, differentiating into hepatic lineage by progressive loss of CK-19/laminin expression and appearance of C/EBP-α expression towards the cluster side. Cells in the epithelial cell adhesion molecule (EpCAM(+)) SHPC clusters showed membranous EpCAM(+)/HNF-4α(+) (hepatocyte nuclear factor-4α) staining and were contiguous to the surrounding cytoplasmic EpCAM(+)/HNF-4α(-) ductular oval cells. Extensive elimination of oval cell response by repeated administration of 4,4'-methylenedianiline (DAPM) to retrorsine-exposed rats impaired the emergence of SHPC clusters.
    CONCLUSIONS:
    These findings highly suggest the hepatic oval cells but not mature hepatocytes as the origin of SHPC clusters in retrorsine-exposed rats.
    PLoS One. 2014 May 5;9(5):e95880.
    Disappearance of GFP-positive hepatocytes transplanted into the liver of syngeneic wild-type rats pretreated with retrorsine.[Pubmed: 24796859]
    Green fluorescent protein (GFP) is a widely used molecular tag to trace transplanted cells in rodent liver injury models. The differing results from various previously reported studies using GFP could be attributed to the immunogenicity of GFP.
    METHODS AND RESULTS:
    Hepatocytes were obtained from GFP-expressing transgenic (Tg) Lewis rats and were transplanted into the livers of wild-type Lewis rats after they had undergone a partial hepatectomy. The proliferation of endogenous hepatocytes in recipient rats was inhibited by pretreatment with retrorsine to enhance the proliferation of the transplanted hepatocytes. Transplantation of wild-type hepatocytes into GFP-Tg rat liver was also performed for comparison. All biopsy specimens taken seven days after transplantation showed engraftment of transplanted hepatocytes, with the numbers of transplanted hepatocytes increasing until day 14. GFP-positive hepatocytes in wild-type rat livers were decreased by day 28 and could not be detected on day 42, whereas the number of wild-type hepatocytes steadily increased in GFP-Tg rat liver. Histological examination showed degenerative change of GFP-positive hepatocytes and the accumulation of infiltrating cells on day 28. PCR analysis for the GFP transgene suggested that transplanted hepatocytes were eliminated rather than being retained along with the loss of GFP expression. Both modification of the immunological response using tacrolimus and bone marrow transplantation prolonged the survival of GFP-positive hepatocytes. In contrast, host immunization with GFP-positive hepatocytes led to complete loss of GFP-positive hepatocytes by day 14.
    CONCLUSIONS:
    GFP-positive hepatocytes isolated from GFP-Tg Lewis rats did not survive long term in the livers of retrorsine-pretreated wild-type Lewis rats. The mechanism underlying this phenomenon most likely involves an immunological reaction against GFP. The influence of GFP immunogenicity on cell transplantation models should be considered in planning in vivo experiments using GFP and in interpreting their results.
    制备储备液(仅供参考)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 2.8458 mL 14.2288 mL 28.4576 mL 56.9152 mL 71.144 mL
    5 mM 0.5692 mL 2.8458 mL 5.6915 mL 11.383 mL 14.2288 mL
    10 mM 0.2846 mL 1.4229 mL 2.8458 mL 5.6915 mL 7.1144 mL
    50 mM 0.0569 mL 0.2846 mL 0.5692 mL 1.1383 mL 1.4229 mL
    100 mM 0.0285 mL 0.1423 mL 0.2846 mL 0.5692 mL 0.7114 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    部分图片展示
    产品名称 产品编号 CAS编号 分子式 = 分子量 位单 联系QQ
    千里(光)非林; Hygrophylline CFN00404 3573-82-8 C18H27NO6 = 353.41 5mg QQ客服:2056216494
    橐吾宁碱; Ligularinine CFN00405 90364-90-2 C18H27NO5 = 337.41 5mg QQ客服:215959384
    倒千里光碱; Retrorsine CFN00407 480-54-6 C18H25NO6 = 351.40 5mg QQ客服:1413575084
    光萼野百合碱; Usaramine CFN00409 15503-87-4 C18H25NO6 = 351.40 5mg QQ客服:3257982914
    光萼野百合碱N-氧化物; Usaramine N-oxide CFN00473 117020-54-9 C18H25NO7 = 367.4 5mg QQ客服:1457312923
    迷迭香宁碱; Rosmarinine CFN00412 520-65-0 C18H27NO6 = 353.41 5mg QQ客服:2159513211
    12-O-乙酰基迷迭香宁碱; 12-O-Acetylrosmarinine CFN00413 137760-53-3 C20H29NO7 = 395.45 5mg QQ客服:3257982914
    Senaetnine; Senaetnine CFN00415 64191-69-1 C20H23NO7 = 389.40 5mg QQ客服:215959384
    千里光宁; Senecionine CFN00417 130-01-8 C18H25NO5 = 335.40 5mg QQ客服:215959384
    全缘千里光碱; Integerrimine CFN00419 480-79-5 C18H25NO5 = 335.40 5mg QQ客服:215959384

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