| Bioresour Technol. 2013 Mar;132:365-9. |
| Purification of fructooligosaccharides by immobilized yeast cells and identification of ethyl β-D-fructofuranoside as a novel glycoside formed during the process.[Pubmed: 23186684] |
A yeast strain (XS1) capable of selective utilization of fructooligosaccharides (FOSs) syrup was identified as Wickerhamomyces anomala.
METHODS AND RESULTS:
Cells of W. anomala XS1 were immobilized in calcium alginate and incubated with an FOS mixture at 30 °C. The purity of the FOS increased from 54.4% to 80.1% (w/w) as 93.6% of monosaccharides were metabolized while the oligosaccharides were not affected. The immobilized yeast cells could be recycled 10 times and the corresponding batch treatments achieved FOS purities around 80%.
CONCLUSIONS:
Thus, the method could be promising for large-scale purification of FOS syrup at low cost. A byproduct formed by the yeast was identified as Ethyl beta-D-fructofuranoside by MS and NMR spectroscopy. |
| J Biosci Bioeng. 1999;87(1):82-6. |
| Use of the yeast Hansenula polymorpha (Pichia angusta) to remove contaminating sugars from ethyl beta-D-fructofuranoside produced during sucrose ethanolysis catalysed by invertase.[Pubmed: 16232429] |
Alkyl glycosides are interesting intermediates for the production of biodegradable surfactants.
Synthesis of Ethyl beta-D-fructofuranoside by invertase-catalysed ethanolysis of sucrose has been extensively reported in literature. However, this procedure yields mixtures of glucose, fructose, sucrose and Ethyl beta-D-fructofuranoside.
METHODS AND RESULTS:
Purification of Ethyl beta-D-fructofuranoside from such mixtures by chromatographic methods is laborious, difficult to scale up and requires organic solvents. The yeast Hansenula polymorpha grows rapidly on glucose, fructose and sucrose. Sucrose hydrolysis in this yeast is catalysed by an intracellular alpha-glucosidase ('maltase'); consequently, H. polymorpha should be unable to hydrolyse Ethyl beta-D-fructofuranoside. Indeed, aerobic cultivation of H. polymorpha on sugar mixtures obtained by invertase-catalysed ethanolysis of sucrose resulted in the complete removal of contaminating sugars, leaving Ethyl beta-D-fructofuranoside as the sole organic compound in culture supernatants.
CONCLUSIONS:
Pure Ethyl beta-D-fructofuranoside was recovered from the supernatants by mixed-bed ion exchange chromatography with an 86% yield. |
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)
