| In vitro: |
| Chinese Journal of New Drugs, 2013(9). | | The in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose based on [Reference: WebLink] | To investigate the in vitro anti-virus effects and dose-effect relationship of epigoitrin and fructopyrano-(1→4)-glucopyranose(FG) from the traditional Chinese medicine Isatidis radix.
METHODS AND RESULTS:
Inhibitory effect of the target components on influenza virus neuraminidase(NA) was determined by fluorescent enzyme immunoassay.The 50% inhibitory concentration(IC50) was used as an indicator to research the relationship between epigoitrin and FG by equivalent curve analysis.The effective concentrations of epigoitrin and FG for in vitro anti-virus activity were determined.In the negative samples,the concentration range of target composition was gradually increased to obtain the key efficacy("incremental").Results: The equivalent point of epigoitrin and FG samples was located below the equivalent line,and the IC50,mix value was less than IC50,add.For anti-influenza A virus activity of the incremental samples of epigoitrin and FG,the effective concentrations were as follows: the lowest,optimum,and highest concentrations of epigoitrin were 82.5,225.0 and 289.7 μg·mL-1;those of FG were 138.2,450.0 and 618.9 μg·mL-1.
CONCLUSIONS:
The equivalent curve analysis shows that epigoitrin and FG exhibit cooperation.We have,for the first time,revealed the effective concentration range for in vitro anti-virus activity of epigoitrin and FG from Isatidis radix. |
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