| In vitro: |
| Planta Med. 2014 Jun;80(8-9):655-61. | | Inhibitory effects of β-chamigrenal, isolated from the fruits of Schisandra chinensis, on lipopolysaccharide-induced nitric oxide and prostaglandin E2 production in RAW 264.7 macrophages.[Pubmed: 24871206 ] |
Much is known about the bioactive properties of lignans from the fruits of Schisandra chinensis. However, very little work has been done to determine the properties of sesquiterpenes in the fruits of S. chinensis.
METHODS AND RESULTS:
The aim of the present study was to investigate the anti-inflammatory potential of new sesquiterpenes (β-Chamigrenal, β-chamigrenic acid, α-ylangenol, and α-ylangenyl acetate) isolated from the fruits of S. chinensis and to explore their effect on macrophages stimulated with lipopolysaccharide. Of these four sesquiterpenes, β-Chamigrenal most significantly suppressed lipopolysaccharide-induced nitric oxide and prostaglandin E2 production in RAW 264.7 macrophages (47.21 ± 4.54 % and 51.61 ± 3.95 % at 50 μM, respectively). Molecularly, the inhibitory activity of β-Chamigrenal on nitric oxide production was mediated by suppressing inducible nitric oxide synthase activity but not its expression. In the prostaglandin E2 synthesis pathway, β-Chamigrenal prevented the upregulation of inducible microsomal prostaglandin E synthase-1 expression after stimulation with lipopolysaccharide. Conversely, β-Chamigrenal had no effect on the expression and enzyme activity of cyclooxygenase-2. In addition, the expression of early growth response factor-1, a key transcription factor of microsomal prostaglandin E synthase-1 expression, was inhibited by β-Chamigrenal.
CONCLUSIONS:
These results may suggest a possible anti-inflammatory activity of β-Chamigrenal which has to be proven in in vivo experiments. | | Arch Pharm Res. 1997 Dec;20(6):633-6. | | Platelet-activating factor antagonistic activity and(13)C NMR assignment of pregomisin and chamigrenal fromSchisandra chinensis.[Pubmed: 18982271 ] | In the course of searching for PAF receptor antagonists, pregomisin (1) and chamigrenal (2) were isolated from the fruits ofSchizandra chinensis Baill by the bioactivity-guided isolation.
METHODS AND RESULTS:
Both compounds showed PAF antagonistic activity and the IC(50) values were 4.8x10(-5) M and 1.2x10(-4) M, respectively. In addition, the(13)C NMR assignments of1 and2 using DEPT, HMQC, COLOC and HMBC were reported for the first time. |
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