| In vitro: |
| Fitoterapia, 1997, 68(4):361-365. | | Analgesic activity of bacosine, a new triterpene isolated from Bacopa monnieri.[Reference: WebLink] |
METHODS AND RESULTS:
Bacosine (1) isolated from the aerial parts of Bacopa monnieri, exhibited moderate analgesic effects but was found to be devoid of effects on barbiturate narcosis, haloperidol-induced catalepsy, spontaneous motor activity (SMA) and conditioned avoidance response (CAR).
CONCLUSIONS:
The analgesic effects, reported for the first time in this plant, were found to be opioidergic in nature. | | Proceedings of the National Academy of Sciences India, 2018, 89(8):1-5. | | In Vitro Analysis of Bacosine as Novel Therapeutic Agent for Murine Breast Cancer.[Reference: WebLink] |
METHODS AND RESULTS:
The anti-metastatic activity of bacosine (Bacopa monnieri plant) was studied using 4T1 murine breast cancer cell line by targeting matrix metalloproteinases (MMPs) which plays important role in invasion and metastasis. The inhibitory concentration (IC50) of bacosine for 4T1 cell line was 21 μM, which indicates that it is toxic to cancer cell line. The effect of different concentrations (10, 21 and 50 μM) of bacosine on metastatic matrix metalloproteinase-9 (MMP-9) gene expression of 4T1 murine breast cancer cells was studied by real time PCR. At high concentrations of bacosine (21 and 50 μM) MMP-9 gene expression was down-regulated after 24 h of treatment in a concentration dependent manner. The effect of bacosine on apoptosis was evaluated by annexin binding assay which showed anti-apoptotic effect in early and late apoptotic stages of cancer cells.
CONCLUSIONS:
These results suggest that bacosine plays a role in regulating cell metastasis by inhibiting MMP-9 and showing anti-apoptotic effect in breast cancer cells. |
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