| In vitro: |
| Applied Microbiology & Biotechnology, 1991, 35(2):216-221. | | Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1 F -fructosyltransferase[Reference: WebLink] |
METHODS AND RESULTS:
Favourable reaction conditions for the enzymatic production of 1-Kestose by sucrose-1F-fructosyltransferase, SFT (EC 2.4.1.99) from Aspergillus phoenicis CBS 294.80 mycelium were established. The intracellular enzyme SFT works best at 60C, exhibits a relatively high thermostability and possesses an alkaline pH optimum. An invertase also present in the mycelium of A. phoenicis possesses an acidic pH optimum.
CONCLUSIONS:
Consequently, around pH 8.0 sucrose is converted mainly to 1-Kestose and nystose while fructose is only formed in relatively small amounts. Under optimal conditions (55 C, pH 8.0 and an initial sucrose concentration of 750 g 1-1) a yield of about 300 g 1-Kestose per 1.01 reaction mixture could be achieved after 8 h. | | International Journal of Food Science & Technology, 2014, 49(6):1500-1505. | | In vitro anti‐hydroxyl radical activity of the fructooligosaccharides 1‐kestose and nystose using spectroscopic and computational approaches[Reference: WebLink] | Fructooligosaccharides (FOS) are bioactive oligosaccharide fructans with beneficial health effects. Anti‐hydroxyl radical activity is an important factor in the description of antioxidant capacity of any natural product. The aim of this study was to estimate in vitro anti‐hydroxyl radical activity of the FOS 1-Kestose and nystose by electron paramagnetic resonance spectroscopy (EPR) and fluorescence spectroscopy (FS) followed by a theoretical approach based on quantum chemistry calculations.
METHODS AND RESULTS:
A significant anti‐hydroxyl radical potential of both compounds was observed (72% and 78% by EPR and 69% and 74% by FS, respectively), indicating the nystose to be a more active natural product. In addition, the computational results have confirmed that nystose follows the same pattern previously shown for 1-Kestose, that is, that carbohydrates can react with hydroxyl radical.
CONCLUSIONS:
It is well known that FOS belong to cardioprotective nutraceuticals, so the study may be of some interest to research in heart disease. |
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